FAM (fluorescein), HEX, JOE, ROX, TAMRA, TET, Texas Red® and others

Background

FAM, JOE, TAMRA and ROX, the "big four" dyes, have dominated DNA sequencing. All four dyes can be excited at a single wavelength (488 nm), but emit at distinctly different wavelengths.

Table 1Selected data for ABI fluorescent dyes

Nameλmax / nm (absorption)λmax / nm (emission)E at λmaxΦτ / ns
FAM™49451868 000--
TET™52153699 000--
JOE™52054875 000--
VIC®538554---
HEX™53555698 000--
NED™546575---
PET®556595--
ROX™57560282 000--
TAMRA™55558065 000--
TET™52153699 000--
Texas Red®59561580 000--