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In convential solid-phase oligonucleotide synthesis, oligos are synthesized with a hydroxyl (OH) group at both the 5′- and the 3′-end. For some applications it is necessary to have a phosphate group at the 5' or 3′-end. (Figure 1).
Figure 1 | Structures of oligonucleotides containing 5′- and 3′-phosphate modifications
Applications in which terminal phosphate groups are used include enzymatic ligation of oligonucleotides and blocking of PCR. For further information see and .
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